Construction vascular-specific expression bi-directional promoters in plants.
Identifieur interne : 003674 ( Main/Exploration ); précédent : 003673; suivant : 003675Construction vascular-specific expression bi-directional promoters in plants.
Auteurs : Xiaomeng Lv [République populaire de Chine] ; Xiaodan Song ; Guodong Rao ; Xiang Pan ; Liping Guan ; Xiangning Jiang ; Hai LuSource :
- Journal of biotechnology [ 1873-4863 ] ; 2009.
Descripteurs français
- KwdFr :
- Fabaceae (génétique), Glucuronidase (génétique), Glucuronidase (métabolisme), Gènes de plante (MeSH), Gènes rapporteurs (MeSH), Histocytochimie (MeSH), Populus (génétique), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Réaction de polymérisation en chaîne (MeSH), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Tabac (génétique), Tabac (métabolisme), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- génétique : Fabaceae, Glucuronidase, Populus, Protéines à fluorescence verte, Tabac, Végétaux génétiquement modifiés.
- métabolisme : Glucuronidase, Protéines à fluorescence verte, Tabac, Végétaux génétiquement modifiés.
- Gènes de plante, Gènes rapporteurs, Histocytochimie, Réaction de polymérisation en chaîne, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Fabaceae (genetics), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Genes, Reporter (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Histocytochemistry (MeSH), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Polymerase Chain Reaction (MeSH), Populus (genetics), Promoter Regions, Genetic (MeSH), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , genetics : Glucuronidase, Green Fluorescent Proteins.
- genetics : Fabaceae, Plants, Genetically Modified, Populus, Tobacco.
- chemical , metabolism : Glucuronidase, Green Fluorescent Proteins, Plants, Genetically Modified, Tobacco.
- Gene Expression Regulation, Plant, Genes, Plant, Genes, Reporter, Histocytochemistry, Polymerase Chain Reaction, Promoter Regions, Genetic.
Abstract
Promoters that have been widely used for both basic research and biotechnological application in plants are generally unidirectional. Here we describe a strategy to bi-directionalize the vascular-specific expression grp1.8 promoter (here named GRPp) and 4CL1 promoter (here named 4CL1p) so that one promoter can direct the vascular-specific expression of two genes, one on each end of the promoter. The minimal promoter (35Smini or GRP mini), when fused at the 5' end of the specific expression promoter (GRPp or 4CL1p) to form bi-directional promoter (35Smini-GRPp, 35Smini-4CL1p or GRPmini-GRPp), was able to direct expression of the glucuronidase (gus) and green fluorescent protein (gfp) gene in all independent transgenic tobacco lines. Stable expression of gusA and gfp genes in transgenic plants was analyzed by histochemical staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The remarkable transcript levels of GFP and GUS were detected by real-time PCR in independent transgenic tobacco lines. Their vascular-specific bi-directional promoters should be used to vascular-specific expression several functional genes in transgenic plants simultaneously.
DOI: 10.1016/j.jbiotec.2009.03.009
PubMed: 19433212
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Xiang" uniqKey="Pan X" first="Xiang" last="Pan">Xiang Pan</name></author><author><name sortKey="Guan, Liping" sort="Guan, Liping" uniqKey="Guan L" first="Liping" last="Guan">Liping Guan</name></author><author><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name></author><author><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19433212</idno><idno type="pmid">19433212</idno><idno type="doi">10.1016/j.jbiotec.2009.03.009</idno><idno type="wicri:Area/Main/Corpus">003568</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003568</idno><idno type="wicri:Area/Main/Curation">003568</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003568</idno><idno type="wicri:Area/Main/Exploration">003568</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Construction vascular-specific expression bi-directional promoters in plants.</title><author><name sortKey="Lv, Xiaomeng" sort="Lv, Xiaomeng" uniqKey="Lv X" first="Xiaomeng" last="Lv">Xiaomeng Lv</name><affiliation wicri:level="3"><nlm:affiliation>College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Song, Xiaodan" sort="Song, Xiaodan" uniqKey="Song X" first="Xiaodan" last="Song">Xiaodan Song</name></author><author><name sortKey="Rao, Guodong" sort="Rao, Guodong" uniqKey="Rao G" first="Guodong" last="Rao">Guodong Rao</name></author><author><name sortKey="Pan, Xiang" sort="Pan, Xiang" uniqKey="Pan X" first="Xiang" last="Pan">Xiang Pan</name></author><author><name sortKey="Guan, Liping" sort="Guan, Liping" uniqKey="Guan L" first="Liping" last="Guan">Liping Guan</name></author><author><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name></author><author><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="eISSN">1873-4863</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fabaceae (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Histocytochemistry (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fabaceae (génétique)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Histocytochimie (MeSH)</term><term>Populus (génétique)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fabaceae</term><term>Plants, Genetically Modified</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fabaceae</term><term>Glucuronidase</term><term>Populus</term><term>Protéines à fluorescence verte</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>Green Fluorescent Proteins</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term><term>Protéines à fluorescence verte</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Genes, Reporter</term><term>Histocytochemistry</term><term>Polymerase Chain Reaction</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term><term>Gènes rapporteurs</term><term>Histocytochimie</term><term>Réaction de polymérisation en chaîne</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Promoters that have been widely used for both basic research and biotechnological application in plants are generally unidirectional. Here we describe a strategy to bi-directionalize the vascular-specific expression grp1.8 promoter (here named GRPp) and 4CL1 promoter (here named 4CL1p) so that one promoter can direct the vascular-specific expression of two genes, one on each end of the promoter. The minimal promoter (35Smini or GRP mini), when fused at the 5' end of the specific expression promoter (GRPp or 4CL1p) to form bi-directional promoter (35Smini-GRPp, 35Smini-4CL1p or GRPmini-GRPp), was able to direct expression of the glucuronidase (gus) and green fluorescent protein (gfp) gene in all independent transgenic tobacco lines. Stable expression of gusA and gfp genes in transgenic plants was analyzed by histochemical staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The remarkable transcript levels of GFP and GUS were detected by real-time PCR in independent transgenic tobacco lines. Their vascular-specific bi-directional promoters should be used to vascular-specific expression several functional genes in transgenic plants simultaneously.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19433212</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2009</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4863</ISSN><JournalIssue CitedMedium="Internet"><Volume>141</Volume><Issue>3-4</Issue><PubDate><Year>2009</Year><Month>May</Month><Day>20</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Construction vascular-specific expression bi-directional promoters in plants.</ArticleTitle><Pagination><MedlinePgn>104-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiotec.2009.03.009</ELocationID><Abstract><AbstractText>Promoters that have been widely used for both basic research and biotechnological application in plants are generally unidirectional. Here we describe a strategy to bi-directionalize the vascular-specific expression grp1.8 promoter (here named GRPp) and 4CL1 promoter (here named 4CL1p) so that one promoter can direct the vascular-specific expression of two genes, one on each end of the promoter. The minimal promoter (35Smini or GRP mini), when fused at the 5' end of the specific expression promoter (GRPp or 4CL1p) to form bi-directional promoter (35Smini-GRPp, 35Smini-4CL1p or GRPmini-GRPp), was able to direct expression of the glucuronidase (gus) and green fluorescent protein (gfp) gene in all independent transgenic tobacco lines. Stable expression of gusA and gfp genes in transgenic plants was analyzed by histochemical staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The remarkable transcript levels of GFP and GUS were detected by real-time PCR in independent transgenic tobacco lines. Their vascular-specific bi-directional promoters should be used to vascular-specific expression several functional genes in transgenic plants simultaneously.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Xiaomeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Xiaodan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>Guodong</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Xiang</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Guan</LastName><ForeName>Liping</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xiangning</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Hai</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>03</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D007887" MajorTopicYN="N">Fabaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006651" MajorTopicYN="N">Histocytochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>12</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>03</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>03</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>5</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>5</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19433212</ArticleId><ArticleId IdType="pii">S0168-1656(09)00132-1</ArticleId><ArticleId IdType="doi">10.1016/j.jbiotec.2009.03.009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Guan, Liping" sort="Guan, Liping" uniqKey="Guan L" first="Liping" last="Guan">Liping Guan</name><name sortKey="Jiang, Xiangning" sort="Jiang, Xiangning" uniqKey="Jiang X" first="Xiangning" last="Jiang">Xiangning Jiang</name><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name><name sortKey="Pan, Xiang" sort="Pan, Xiang" uniqKey="Pan X" first="Xiang" last="Pan">Xiang Pan</name><name sortKey="Rao, Guodong" sort="Rao, Guodong" uniqKey="Rao G" first="Guodong" last="Rao">Guodong Rao</name><name sortKey="Song, Xiaodan" sort="Song, Xiaodan" uniqKey="Song X" first="Xiaodan" last="Song">Xiaodan Song</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lv, Xiaomeng" sort="Lv, Xiaomeng" uniqKey="Lv X" first="Xiaomeng" last="Lv">Xiaomeng Lv</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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